This project involves the development of strategies to investigate microorganism-dye interactions using microfluidic devices. This project expands on the staining kinetics studies, previously done by others in the Vullev group, by incorporating the immobilization of bacterial cells and/or spores within microfluidic devices in order to image and track the progress of fluorescence intensity enhancement at the single cell level.
Conventional approaches of fabricating microelectrodes are known to produce harmful products for the environment. This project provides an alternate, more environmentally benign, method of fabricating electrically conductive microelectrodes using enzymatic production of polyaniline. Furthermore, these microelectrodes can be used in biosensing applications using impedence spectroscopy.
