We develop bioactive interfaces through chemical derivatization of the surfaces of silica-based substrates. The surface layers are composed of amphipathic polymers that suppress nonspecific interactions and provide bioinert (or nonfouling) qualities of the interfaces. Furthermore, such bioinert layers do not compromise the properties of biological molecules attached to them. By the attaching of selected proteins to the nonfouling interfaces, we control their functionality, exclusively allowing the targeted biospecific interactions and suppressing the undesired nonspecific adsorption.
Literature Reference:
Wan, Jiandi; Thomas, Marlon S.; Guthrie, Sean; Vullev, Valentine I. "Surface-Bound Proteins with Preserved Functionality," Ann. Biomed. Eng. 2009, 37, 1190–1205.
